Journal: bioRxiv

Article Title: Integrative systems neuroimmunology reveals leukocyte-expressing PAX6 as a critical predictor of major depressive disorder

doi: 10.1101/2024.09.25.614771

Figure Lengend Snippet: ( a-c ) Flow cytometry analysis shows the identification of four distinct immune cell clusters based on the expression of CD45, CD3, CD4, CD11b, CD19, and Ly6C. ( c-f ) Detailed analysis of Cluster 4, highlighting the upregulation of PAX6 expression within immune cells following CRS. The Fig. illustrates the immunomodulatory effects of CRS, with an increase in PAX6 expression and the expansion of myeloid and plasma cell populations (CD11b and Ly6C), as well as a reduction in T cell markers (CD3). N=7 controls and 5 stressed. For ( f ), student’s t-test was used. *p<0.05, **p<0.01, ****p<0.0001.

Article Snippet: Cells were then stained for flow cytometric analyses using the following dyes/antibodies: fixable viability dye Zombie Aqua (1:1000; BioLegend; cat.# 423101) to exclude dead cells, BUV395-anti-CD45 (BD Biosciences; 1:200; cat.# 564616), BUV737-anti-CD3 (BD Biosciences; 1:200; cat.# 612771), PE-Cy7-anti-TCRb (Biolegend; 1:200; cat.# 109222), BV421-anti-TCRgd (Biolegend; 1:200; cat.# 118120), BUV496-anti-CD4 (BD Biosciences; 1:300; cat.# 612952), BV711-anti-CD8 (Biolegend; 1:300; cat.# 100748), AF700-anti-CD19 (Thermo Fisher Scientific; 1:300; cat.# 56-0193-82), APC-anti-CD11b (Biolegend; 1:100; cat.# 101212), BV785-anti-Ly6C (Biolegend; 1:100; cat.# 128041) and AF488-anti-Pax6 (Bioss USA; 1:100; cat.# bs-11204R).

Techniques: Flow Cytometry, Expressing